human prostate carcinoma cell line du145  (ATCC)

Journal: Cell Reports Methods

Article Title: Biobank of genetically defined murine prostate cancer tumoroids uncovers oncogenic pathways and drug vulnerabilities driven by PTEN-loss

doi: 10.1016/j.crmeth.2026.101370

Figure Lengend Snippet: The PDPK1/AKT/FLT DPI and tenovin-6 (T6) show high anti-cancer efficacy in murine tumoroids and human PCa cell lines (A) Dose-response curves for DPI (top) and T6 (bottom) for in vivo and in vitro Pten KO (left), Pten/Stat3 KO (middle), and Pten/Tp53 KO (right) tumoroids. Points represent means of technical duplicates per tumoroid line ( N = 3). Curve fitting was performed using GraphPad Prism 8.0.2. (B) Bar graphs showing means and ±SD of half-maximal inhibitory concentration (IC50) for DPI (top) and T6 (bottom) for in vivo and in vitro tumoroid lines of all genotypes ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA, Tukey’s test). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05. (C) Bar graphs depicting means and ±SD of IC50 values of DPI (left) and T6 (right) on human PCa cell lines. 22RV1: primary PCa; LNCaP: metastatic PCa; DU145, PC3: metastatic castration-resistant PCa ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05; ∗∗ p ≤ 0.01. (D) Heatmaps of synergy scores calculated with the highest single agent (HSA) model for DPI and enzalutamide (left), and T6 and enzalutamide (right) on the human LNCaP cell line. Values > 0 represent synergistic effects, and values < 0 represent antagonistic effects. IC50 concentrations of respective compounds are underlined ( N = 3). See also .

Article Snippet: Human DU145 metastatic PCa cell line , ATCC , HTB-81, RRID:CVCL_0105.

Techniques: In Vivo, In Vitro, Concentration Assay

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Schematic of hedgehog-mediated abirateronylation as a noncanonical PTM (ncPTM) for generating protein–drug conjugates (PDCs). An intrinsically disordered protein polymer (IDPP) serves as the model protein scaffold. The resulting hybrid biopolymers are internalized by DU145 cells and exert cytotoxicity. Created with BioRender.com

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Polymer

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Biophysical and biological assessment of the optimized PDC and controls. (a) Hydrodynamic radius ( R h ) of (A,40) carrier (E), E–Abi and E–Chol at 50 μM and 200 μM, measured by dynamic light scattering. E–Abi forms reversible, concentration-dependent oligomers. (b) Release kinetics of conjugated sterols (E-Abi, E–Andro and E–Chol) in PBS (pH 6.5) at 37 °C, along with the chemical structures of Abi and Andro tail groups. E–Abi and E–Andro shows sustained release, whereas E–Chol displays negligible sterol release over the same period. Shaded areas represent the 95% confidence intervals of the nonlinear regression fit to a first-order kinetics model. (c) Dose–response curves for DU145 prostate cancer cells after 72 h exposure to free drug, conjugate, and controls (measured by MTT assay; nonlinear dose–response fit). (d) Viability of 3D DU145 spheroids after 24-h treatment with 100 μM of each compound, showing superior efficiency of E–Abi. (e) Representative live/dead fluorescence images of treated spheroids. Live cells are stained green (calcein AM), and dead cells are stained red (propidium iodide). Data are mean ± s.d. ( n = 3). Statistical analysis was performed using one-way ANOVA with posthoc tests (Tukey’s for a, Holm–Sidak’s for d): * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Concentration Assay, MTT Assay, Fluorescence, Staining

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Lipidation-dependent modulation of biopolymer penetration in 3D tumor spheroids. (a–c) Representative bivariate flow cytometry dot plots of WGA-Alexa Fluor 680 (membrane label) versus carrier-Alexa Fluor 488. The gated population represents WGA + /Carrier + cells, and the percentage of uptake-positive cells is indicated for each condition. DU145 spheroids were incubated overnight with labeled (a) E, (b) E–Abi, (c) E–Chol, dissociated to single cells, and analyzed by flow cytometry. (d) Bar graph summarizing the fraction of uptake-positive cells across treatments. (e–g) Confocal z-stack images of DU145 spheroids treated with E, E–Abi, or E–Chol for 24 h. WGA (AF680, red) marks the cell membrane, and constructs (AF488, green) indicate carrier localization. Data are mean ± s.d. ( n = 3). Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparisons test (** p < 0.01).

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Flow Cytometry, Membrane, Incubation, Labeling, Construct

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Membrane, Fluorescence, Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: Bio-protocol

Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants

doi: 10.21769/BioProtoc.5614

Figure Lengend Snippet: . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.

Article Snippet: Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.

Techniques: Activation Assay, Control, Derivative Assay, Expressing